![]() Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance. It is often referred to as the “stress hormone” as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. After a short incubation the color reaction is read at 450nm.Ĭortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. The substrate reacts with the bound cortisol-peroxidase conjugate. After an 1 hour incubation the plate is washed and substrate is added. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol standard is provided to generate a standard curve for the assay. This kit measures total cortisol in extracted samples, serum and plasma and free cortisol in saliva and urine. ![]() The DetectX ® Cortisol Enzyme Immunoassay Kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and culture media samples. Abbkine Protein Labelling & Quantification.TargetMol Chemicals Neuroscience Products.TargetMol Chemicals Microbiology products.TargetMol Chemicals Immunology Products.Leading Biology SARS-CoV-2 Recombinant Protein.Leading Biology Rabbit Anti-Rat Antibodies.Leading Biology Rabbit Anti-Mouse Antibodies.Leading Biology Rabbit Anti-Human Antibodies.Leading Biology DNA & RNA Detection & Purification.Agrisera Plant Biology & Photosynthesis Product.Affinity Biosciences Rabbit Anti-Human Antibodies.Affinity Biosciences Primary Antibodies.Affinity Biosciences Polyclonal Antibodies.Affinity Biosciences Monoclonal Antibodies.
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